Qiagen
QIAquick PCR Purification Kit
28104
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I have used the Qiagen QIAquick PCR Purification kit to successfully purify DNA from PCR products, thus removing primers, nucleotides, polymerases etc. using the QIAquick spin columns in a microcentrifuge. These products were suitable for downstream Sanger sequencing.
PCR product cleanup for downstream Sanger sequencing
PCR products
The protocol is quick and easy to follow and makes use of spin-columns. Briefly, the microcentrification method involves addition of Qiagen supplied buffer to the sample which is then applied to a spin column. The DNA binds to the column while impurities are removed via wash steps using the Qiagen supplied buffer. The purified DNA is then eluted into elution buffer or water.The protocol can be adapted to use with a microcentrifuge or vacuum manifold.
The initial buffer works at an optimal pH and the kit includes an optional pH indicator which can be added to this buffer.
Purified DNA is suitable for downstream Sanger sequencing.
None
Rapid and effective kit to purify PCR products
Kit is relatively expensive
Effective kit for the efficient removal of contaminants from PCR products prior to Sanger sequencing.
Product Name: QIAquick PCR Purification KitSupplier Page