EMD Millipore
Anti-Ubiquitinylated proteins Antibody, clone FK2
04-263
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Browse Similar Products: Anti-Ubiquitinylated Proteins Immunoprecipitation Antibodies
Ubiquitination is the most important way to regulate proteins in cells. Our lab has demonstrated that FbxL5 can direct SNA1 to degradation thought its polyubiquitination. We use this commercial antibody to detect ubiquitination of proteins during our experimental procedures.
Immunoprecipitation
HEK293T cells were transfected with pcDNA3-Snail1-HA and Myc-FBXL5 (L5) for 24 h and treated with 10 mM MG132 for 3 h
1/50
Not needed
Anti Ms magnetic beads
WB
Nice detection of poli- Ubiquitinated proteins (SNA1 in this case). Also mono-Ubi is detected (not shown)
Specificity.
Any remarkable.
Good option to detect Ubiquitinated proteins.