Life Technologies
Anti-V5 Antibody
37-7500
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Protein tagging is a very usual method to detect proteins with no available good commercial antibodies. V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5) and can be used as most common tags (HA, Myc or FLAG)
Western Blot
Human cells MCF7
1/5000
Milk 5% in TBS-T
Anti Ms HRP
Not needed
ECL
In this case different amounts of transfected Twist - V5 are detected.
Specific signal, strong signal
Any remarkable.
Any nonspecific band is detected, as desired in an anti-tag antibody.