Excellent Antibody for T-Cell Activation

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Quality of Results

Ease-of-Optimization

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Biology
California Institute of Technolgy
Post-doctoral research scholar

Company:

eBiosciences

Product Name:

Anti-human CD3 FG Purified OKT3 50 ug

Catalog Number:

16-0037-81

Info: View Product View Product Specs

Browse Similar Products: CD3 Antibodies

My research focuses on T cell based immunotherapy. For this, I regularly activate and expand primary T cells from PBMCs. Anti-CD3 antibody is a very potent tool to activate T cells (in conjunction with Anti-CD28), in a non-antigen specific manner.

Experimental Design and Results Summary

Applications

Other

Sample

Primary human PBMCs

Primary Incubation

3-7 days

Blocking Agent

None

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

NA

Results Summary

When the antibody was used in conjunction with IL-2 and anti-CD28 antibody, ~90% of the cells at day 7 were CD3+ T cells. A similar enrichment of T cells is also obtained by using just anti-CD3 and IL-2.These results show that anti-CD3 antibody from eBiosciences is highly effective at selectively expanding CD3+ T cells from PBMCs.

Additional Notes

None

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Summary

The Good

Very robust activation of T cells from PBMCs

The Bad

Nothing

The Bottom Line

One of the best reagents to activate T cells

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