DakoCytomation
Rabbit anti-GFAP
Z0334
Used in experiments aimed at determining astrocyte cell numbers in the brain of postnatal mice undergoing hypoxic insult. GFAP is a well-known marker for astrocytes.
Immunofluorescence
Floating brain tissue fixed in 4% PFA and cryosectioned to 50um thickness
1:1000 dilution in blocking agent at 4C overnight
5% Goat Serum; 0.5% Triton X-100; PBS
1:1000 dilution of Goat ant-Rabbit Alexa Fluor IgG in PBS
None
Fluorescence detected via epifluorescent microscopy
Obtained fluorescent images that could be analyzed using stereological software.
Antibody is robust, specific, and gives a great signal.
Great, specific antibody for detection of astrocytes in fixed brain tissue.