BioLegend
PE anti-human CD14 Antibody
325606
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CD14 is the LPS receptor, expressed at high levels on monocytes. When staining ficoll-paque isolated human PBMC, the monocytic gate is not always accurate enough to identify monocytes; hence it is useful to stain for CD14. We selected this product because it fits well with the other antibodies and fluorochromes used.
Flow Cytometry
Human PBLs
5 microlitre antibody for 30 minutes on ice (recommeded by BioLegend)
Human IgG 1ug/ml solution
Not used
Flow cytometry
The monocytes stained very brightly with CD14; however, despite a 4-fold dilution (using a forth of the recommended antibody volume), we still found some background signal. Also, the signal on monocytes got weaker with higher antibody dilutions.
Events shown are from a combined lymphocytes and monocytes gate.
Strong signal
High background
A good antibody, but the high background lets it down.