LC3B Antibody for Tissue Staining

Overall

Quality of Results

Ease-of-Optimization

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biology
Whitehead Institute for Biomedical Research
postdoctoral associate

Company:

Cell Signaling Technology

Product Name:

LC3B (D11)

Catalog Number:

#3868

Info: View Product View Product Specs

Browse Similar Products: LC3B IHC Antibodies

I have tried several antibodies to stain xenograft tisse for LC3B- a marker for autophagososmes. The cell signaling LC3B D11 antibody works great with almost no background, very specific staining. It works great for western blotting too.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Xenograft tumor tissue

Primary Incubation

1:3000, RT 1 hr or overnight 4C

Blocking Agent

BSA

Secondary Incubation

Anti rabbit-HRP, RT 1hr

Tertiary Incubation

None

Detection

DAB

Results Summary

I have tried several antibodies to stain xenograft tisse for LC3B- a marker for autophagosomes. The cell signaling LC3B D11 antibody works great with almost no background, very specific staining. It works great for western blotting too.

Additional Notes

None

Related Categories

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Summary

The Good

Clean, no background

The Bad

None

The Bottom Line

Great product

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