Very Good: Anti-ATRX, Immunofluorescence

March 31, 2014

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Biological Science
Florida State University
Assistant Professor

Company:

Santa Cruz Biotech, Inc

Product Name:

Anti-ATRX (C-16) Goat pAb

Catalog Number:

Sc-10080

The purpose of this experiment was to identify an antibody that was suitable for detection of ATRX in human cells by indirect immunofluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human Hela cells

Primary Incubation

1:200 at room temperature for 60 minutes. Staining was performed in 1 x PBS and 0.1% Tween 20

Blocking Agent

Slide was pre blocked with 3% BSA in 1 x PBS and 0.1% Tween 20

Secondary Incubation

1:100 dilution of Rhodamine Donkey anti-goat secondary diluted in 1 x PBS and 0.1% Tween-20 for 60 minutes at room temperature

Tertiary Incubation

No tertiary incubation was performed

Detection

Direct labeled donkey anti-goat antibodies

Results Summary

This antibody stains well and gives the expected nuclear distribution and intense speckles for ATRX as has been shown with several other independent antibody sources.

Additional Notes

Cells were grown directly on slides and washed briefly with 1 x PBS before fixing and extracting for 10 minutes at room temperature in 4% formaldehyde, 1 x PBS and 0.1% Triton-X100

Image Gallery

Summary

The Good

Very good antibody for immunofluorescence.

The Bad

No drawbacks were evident with this antibody

The Bottom Line

SCBT provides 1.0ml of antibody which is plentiful for countless staining experiments. Ours is 12 years old and still works well.

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