Clathrin Heavy Chain Antibody

March 10, 2014

Overall

Quality of Results

Ease-of-Optimization

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biology
Whitehead Institute for Biomedical Research
postdoctoral associate

Company:

Cell Signaling Technology

Product Name:

Clathrin heavy chain antibody

Catalog Number:

D3C6

A clean antibody to detect Clathrin heavy chain. High specificity but sensitivity is okay, load 30-40 ug total protein to get a strong signal.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cells

Primary Incubation

1:1000, 4C, overnight

Blocking Agent

5% milk-TBST

Secondary Incubation

Anti-rabbit HRP, 1 Hr, room temperature

Tertiary Incubation

None

Detection

Chemiluminescence

Results Summary

Western blot showing Clathrin heavy chain expression in three different breast cancer lines.

Additional Notes

None

Image Gallery

Summary

The Good

Very specific

The Bad

None

The Bottom Line

Great antibody.

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