Product Reviews

CD3, CD4, CD8 Antibodies

BioLegend

March 03, 2014

These antibodies from BioLegend work extremely well. I used these to phenotype in vitro expanded primary T cells. These antibodies give me ... Read Review

Reliable CD103 Antibody

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BioLegend

March 03, 2014

The aim of the research is to study splenic hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with... Read Review

Maximizing Double Stranded RNA Synthesis

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Thermo Fisher Scientific

February 28, 2014

In vitro transcription of RNA transcripts has become an important laboratory technique for many cell and molecular biology experiments. The ... Read Review

CD206 (MMR) Antibody: Works Out of the Box

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BioLegend

February 28, 2014

The aim of the research is to study splenic hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with... Read Review

CD11c Antibody: Reliable

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BioLegend

February 27, 2014

The aim of the research is to study lymph node myeloid cells in cancer. In my experiment, I tested if these myeloid cells are altered in ... Read Review

Transit-293 Transfection Reagent

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Mirus Bio

February 27, 2014

This experiment was a first-time test of the efficacy of Transit-293 Read Review

LC3B Antibody for Tissue Staining

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Cell Signaling Technology

February 26, 2014

I have tried several antibodies to stain xenograft tisse for LC3B- a marker for autophagososmes. The cell signaling LC3B D11 antibody works ... Read Review

Excellent IL-23 ELISA Product

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BioLegend

February 25, 2014

We are trying to determine the levels of overexpressed IL-23 from the minicircle that we inject in the mice. Although the components for the... Read Review

PrimeSTAR GXL DNA Polymerase

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February 20, 2014

For this project, my goal was to use a PCR-based strategy to genotype C. elegans strains in order to confirm the presence or absence of ... Read Review

Source Bioscience Sanger Sequencing Service

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February 19, 2014

I have used Source Bioscience for Sanger sequencing of pre-purified PCR products. I took advantage of the free collection service and the ... Read Review

Pacific Blue Anti-mouse F4/80

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BioLegend

February 18, 2014

The goals are to determine what are the mechanisms for inflammation-induced insulin resistance. Read Review

MPM-2 - Cell Cycle Analysis by IF

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DAKO Corporation

February 17, 2014

MPM-2 antigens are strongly expressed within mitotic cells. In eukaryotes, MPM-2 phosphoproteins have been found in the cytoplasm of ... Read Review

rIl-33 for in vitro and in vivo Usage

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BioLegend

February 10, 2014

We used rIl-33 from Biolegend and it works very well both in culture set up and in vivo. Read Review

Great Cleaved Caspase 3 Antibody

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Cell Signaling Technology

February 07, 2014

Experiments were aimed at detecting apoptotic cells in postnatal mouse brain tissue. Cleaved Caspase 3 is a well-known marker for cells ... Read Review

DNA/RNA/Protein Extraction Kit

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February 07, 2014

Macherey Nagel TriPrep kit is intended to extract DNA/RNA/protein in a single protocol. This is highly needed and desired to get consistent... Read Review

SapphireAmp Fast PCR Master Mix

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February 06, 2014

This reagent is designed to rapidly set up a hot start PCR. This made it ideal for large scale genotyping to assess a single nucleotide ... Read Review

Great KI67 antibody

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February 06, 2014

Used in experiments aimed at determining proliferating cell populations in the the brain of postnatal mice. KI67 is a marker for cells that ... Read Review

Great GFAP Antibody

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DakoCytomation

February 05, 2014

Used in experiments aimed at determining astrocyte cell numbers in the brain of postnatal mice undergoing hypoxic insult. GFAP is a well-... Read Review

Counting cells with NC3000

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February 04, 2014

We are counting our cells with NC3000 it provides cell viability, % aggregates. The instruments provides very robust counts regardless cell ... Read Review

PCR Product Cleanup

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QIAGEN - Sample to Insight

January 31, 2014

I have used the Qiagen QIAquick PCR Purification kit to successfully purify DNA from PCR products, thus removing primers, nucleotides, ... Read Review

Norgen Biotek Total RNA Purification Kit

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Norgen Biotek Corporation

January 30, 2014

I am interested in the microRNA expression of exosomes isolated from human plasma. I have used this kit to extract RNA from exosomes ... Read Review

CD45R/B220 Antibody

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BioLegend

January 29, 2014

Study bone marrow hemopoietic progenitor cells Read Review

E-Cadh as Epithelial Marker

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January 28, 2014

The CDH1 gene provides instructions for making a protein called epithelial cadherin or E-cadherin. Cadherins are a group of proteins on the ... Read Review

Ok CD3 Antibody

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BioLegend

January 28, 2014

Study bone marrow hemopoietic progenitor cells Read Review

Loading Control for WB: Tubulin

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January 27, 2014

Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha... Read Review

Brilliant CD183 (CXCR3) Antibody

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BioLegend

January 27, 2014

Study bone marrow hemopoietic progenitor cells Read Review

Alpha Amylase Detection in Pancreas

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January 24, 2014

a-Amylase is a protein enzyme EC 3.2.1.1 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, ... Read Review

CD150 (SLAM) Antibody

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BioLegend

January 24, 2014

Study bone marrow hemopoietic progenitor cells Read Review

PTEN detection by WB

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Cell Signaling Technology

January 23, 2014

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) ... Read Review

Sca-1 Antibody: Works Out of the Box

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BioLegend

January 23, 2014

Study bone marrow hemopoietic progenitor cells Read Review

Excellent HSP90 Antibody for Western Blot

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Santa Cruz Biotechnology, Inc.

January 22, 2014

I used this antibody as loading control. It gave a very specific band at the right size with no background or unspecific band. Read Review

Fast, Easy and Reliable Cell Counting

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January 22, 2014

Our group mainly use the NC-200 for viability checks and cell counting in primary cultures. All is done in one step. Due to FACS-style ... Read Review

Akt Pathway Activation Analysis

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Cell Signaling Technology

January 22, 2014

Since its initial discovery as a proto-oncogene, the serine/threonine kinase Akt (also known as protein kinase B or PKB) has become a major ... Read Review

Akt Detection in MDCK Cells

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Cell Signaling Technology

January 21, 2014

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by ... Read Review

Cells-to-cDNA Kit: Not Easy as Promised

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Thermo Fisher Scientific

January 21, 2014

Cells-to-cDNA kit is designed to minimise steps in carrying out conventional end point PCR. It has two options, One step and two step RT-PCR... Read Review

HNRNPC Antibody in A549 Using Western Blot

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Aviva Systems Biology

January 20, 2014

HNRNPC - Western Blot detection in A549 (human Aveolar Basal Epithelial Cells) Read Review

GFP Detection by WB

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Abcam

January 20, 2014

GFP is a fluorescent protein that is used as protein tag in vitro. Its detection by WB can be used to normalize transfection or to detect ... Read Review

ITGB3 Antibody in A549 Cells Western Blotting

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Aviva Systems Biology

January 17, 2014

ITGB3 antibody – in A549 cells - human Aveolar Basal Epithelial Cells - Western Blotting Read Review

Cell Cycle and CDC25A

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Santa Cruz Biotechnology, Inc.

January 17, 2014

The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the ... Read Review

CLK1 Antibody Human Microvascular Endothelial Cells Western Blotting

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Aviva Systems Biology

January 16, 2014

Western Blotting with CLK1 antibody - N-terminal region (ARP52021_P050) in HMEC-1 Read Review

Cell Adhesion and E-Cadherin Antibody Review

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January 16, 2014

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting ... Read Review

CLK4 Antibody HMEC-1 Western Blotting

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Aviva Systems Biology

January 15, 2014

Wetsern Blot with Clk4 antibody in HMEC-1 Read Review

Great PD-1 for Flow Cytometry

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BioLegend

January 15, 2014

I work in a murine system to characterize antiviral responses when the animals are exposed to exogenous immunological insults. I have used a... Read Review

14-3-3 sigma/Stratifin Antibody for Flow Cytometry

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Novus Biologicals, a Bio-Techne Brand

January 10, 2014

14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been ... Read Review

CellROX Green - ROS Detection Kit

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January 09, 2014

ROS production after cell cycle arrest. Ready-to-use kit, there is no optimalisation needed for single colour staining (we did not even need... Read Review

Excellent Anti Phospho ERK1/2 Antibody for WB

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Cell Signaling Technology

January 08, 2014

The goal was to test ERK1/2 activation in primary mouse cardiac cells after growth factors (FGF, PDGF and EGF) stimulation. As shown on the ... Read Review

ICC for CRH Receptor Type 1

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January 07, 2014

Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and ... Read Review

Great Anti MEK1 Antibody

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Abcam

January 03, 2014

I needed a very specific antibody to test the genetic deletion of MEK1 in the mouse heart using Western blot. I selected an antibody from ... Read Review

SensiFAST Probe Hi-ROX real-time PCR master mix

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Bioline

January 03, 2014

I am interested in the expression of microRNAs as biomarkers for onset and severity of graft versus host disease (GvHD). I frequently ... Read Review

CONFIRM anti-LMO2 mouse monoclonal antibody (1A9-1)

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Roche

January 02, 2014

I am interested in the prognostication of an aggressive B-cell lymphoma called diffuse large B-cell lymphoma (DLBCL). In order to stratify ... Read Review

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