The Standard for Comparing All Other PCR Instruments

Overall

Performance

Ease-of-Optimization

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Rice University
BioEngineering
Researcher

Company:

Roche Applied Science

Product Name:

Roche LightCycler480

Catalog Number:

05015278001

Easy enough to train undergrads on, but powerful enough for a senior research scientist, the Roche LightCycler 480 is the gold standard I use to compare all other PCR instruments to for gene quantification and endpoint genotyping. Fantastic sensitivity and reproducibility both with our in-house primers and when testing other commercial sets when paired with SYBR Green I. Extensive room for expansion with multicolor assay options and high throughput capabilities. It is a high initial investment and has some consumables, but for our lab it's worth the price for high quality results.

Experimental Design and Results Summary

Tips

The usual qPCR advice- aliquot primers, equal reaction volumes, spin the plate before running, and validate validate validate

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Summary

The Good

Powerful, high-sensitivity results with high reproducibility

The Bad

Initial investment

The Bottom Line

Worth it, I never want to go back to our old qPCR instrument.

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