Roche Applied Science
Roche LightCycler480
05015278001
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Easy enough to train undergrads on, but powerful enough for a senior research scientist, the Roche LightCycler 480 is the gold standard I use to compare all other PCR instruments to for gene quantification and endpoint genotyping. Fantastic sensitivity and reproducibility both with our in-house primers and when testing other commercial sets when paired with SYBR Green I. Extensive room for expansion with multicolor assay options and high throughput capabilities. It is a high initial investment and has some consumables, but for our lab it's worth the price for high quality results.
The usual qPCR advice- aliquot primers, equal reaction volumes, spin the plate before running, and validate validate validate
Powerful, high-sensitivity results with high reproducibility
Initial investment
Worth it, I never want to go back to our old qPCR instrument.
Product Name: LightCycler®480 Instrument IISupplier Page