Abcam
Anti-P Glycoprotein antibody
ab170904
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The longstanding goal in our lab is to understand how drug resistance develops. One of the known methods of developing tumor resistance is via the expression of PGP. Here we test if the antibody can detect PGP in colon cancer cells using immunofluoroscence. From the results it is evident that the antibody works very well to detect PGP expression in colon epithelial cells on the surface.
Immunofluorescence
Human colon cancer cell line
1:500 in block buffer
Fish gelatin or 0.5% FBS in PBS
Alexa Fluor 405
Nucleus stain
Using Zeiss confocal microscope
Green color indicates PGP expression, however a cell surface marker is required to distinguish from cell surface.
None
Very clean antibody, no background
None observed
Good to detect PGP using IFC