Facilitated RNA Extraction

July 31, 2015

BlueskyReddit
Northwestern
Neurological Surgery
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

RNeasy Minikit

Catalog Number:

74104

Extracting RNA from cell lines or from tissue specimens has the historic connotation of being an arduous process. This kit, from Qiagen, makes RNA extraction easy with pure RNA content after extraction. The reagents are inclusive within the kit and it takes roughly 30 minutes to extract RNA from cell lines with repeatable purity values.

Experimental Design and Results Summary

Application

RNA

Starting Material

Glioblastoma cells

Protocol Overview

Protocol was followed verbatim: Initially adherent cells were trypsinized and pelleted. The cell pellet was resuspended in fresh media. The specimen was then re-centrifuged and the protocol was followed thereafter.

Tips

Requires absolute ethanol. Would spin a minute longer to dry the membrane towards the end of the protocol.

Results Summary

RNA analysis demonstrated a pure specimen. The RNA extracted was utilized for RT-PCR/qPCR analysis of gene expression of glioblastoma cells.

Additional Notes

None

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Summary

The Good

Reliable purity, high RNA concentration

The Bad

Kit pricey for few reactions

The Bottom Line

Overall, the kit provides reliable, easy, and fast RNA extraction from cell lines. Some drawbacks include the price for the number of reactions. Moreover, the mini-kit is bottlenecked by the amount of RNA concentrations extracted per sample. Nonetheless, this kit is at the top for RNA extraction from cultured cells/tissue specimens.

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