Time Course Effect of MCP-1 Induced Phosho p70S6 Kinase in HASMS

August 07, 2015

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling Technology

Product Name:

p70S6 kinase

Catalog Number:

9204

The aim of this study is to check the MCP-1 induced pp70S6K role in human aortic smooth muscle cells (HASMS). These p70S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. In the current study we want to elucidate its role in HASMS cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cell line

Primary Incubation

1:1000 in 5% BSA

Blocking Agent

5% BSA from Sigma prepared in 1X TBST

Secondary Incubation

1:4000 in 5% BSA

Tertiary Incubation

NA

Detection

GAR

Results Summary

It doesn't have any change in the phosphorylation levels by MCP-1

Additional Notes

None

Image Gallery

Summary

The Good

This antibody is very specific and I didn't see any background..

The Bad

The signal is not as strong as expected and we have wait for at least 15 minutes to get the signal.

The Bottom Line

This antibody is very good for Western blot, it is very specific and without much background. The signal can be detectable even at low concentration. It is definitely recommended for performing Western blot.

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