Ninja TAQ Found be a Very Robust and Sensitive Enzyme for Amplification

University of Colorado, Denver
School of Pharmacy
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Company:

Pharmozyme

Product Name:

Ninja TAQ Hot Start Polymerase DNA

Catalog Number:

Ninja TAQ

This enzyme was found to be better than any other commercially available TAQ polymerase. It works well for 200bp product amplification. Also there is no need for hot start.

Experimental Design and Results Summary

Application

cDNA amplification

Starting Material

200ng cDNA

Protocol Overview

250nM primer concentration, 200ng cDNA in 15ul reaction.

Tips

Use minimum quantity of enzyme

Results Summary

Got very distinct bands on 2% agarose gel without any non-specific product band.

Additional Notes

None

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Summary

The Good

Specificity and stability

The Bad

Price

The Bottom Line

I recommend this enzyme to use whenever you are amplifying a product from 200-1000bp.

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