FBRS Antibody

August 07, 2015

National institute of Dental and Craniofacial Research
national institutes of health
Post doctoral fellow

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Company:

Novus Biologicals

Product Name:

FBRS

Catalog Number:

NBP1-83917

Info: View Product View Product Specs

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Our current project is all about identification of affected proteins and their levels upon specific treatment of mammalian (HeLa) cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Untreated and treated Hela cell lysate 25 ug protein

Primary Incubation

1:500 overnight at 4 degrees Celsius

Blocking Agent

Blocker=Skimmed milk, Buffer= 1XTris Buffer Saline+0.1 % Tween 20, 2hrs at RT

Secondary Incubation

1:20,000 HRP-conjugated secondary, 60 minutes at room temp

Tertiary Incubation

NA

Detection

Femto Chemiluminescent

Results Summary

This antibody gives a single major band at ~90 kDa and one more faint band about ~50 kDa

Additional Notes

None

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Summary

The Good

The good thing is that at least you see your desired band

The Bad

The bad part is you should not get confused with the intense faint other band

The Bottom Line

If you have any other alternatives to this antibody, I would suggest you try that first.

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