Great Antibody for Gamma Delta Cells

Neurology
BWH
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse gamma delta TCR PE

Catalog Number:

12-5711-82

Info: View Product View Product Specs

Browse Similar Products: TCR Antibodies

We are interested in the contribution of gd T cells in the induction of tolerance. We choose most of our FACS antibodies from eBioscience for their good price-quality ratio. The best results were obtained with the clone eBioGL3 in PE and APC.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen cells

Primary Incubation

20min 4'C

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS LSRII

Results Summary

For the detection of gamma delta T cells, a double staining for CD3 and gd TCR is required. This anti-mouse gamma delta TCR PE gave very bright and specific staining.

Additional Notes

We used a dilution of 1/200 and 50ul per sample

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Summary

The Good

Bright staining

The Bad

Don't forget to combine with CD3 staining

The Bottom Line

Great antibody for detection of mouse gamma delta T cells

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