Santa Cruz Biotechnology
ADAM10 Antibody (B-3)
sc-28358
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The aim of the present experiment was to test the time course effect of VEGFD on ADAM10 expression in human endothelial cells. We have selected this antibody from Santa Cruz Biotechnology because it detects ADAM10 endogenous levels in mammalian cell lines.
Western Blot
Whole cell lysate from human endothelial cell
1:500 (5%BSA in TBST), Overnight at 4 degrees Celsius
5% Skimmed milk in TBST
1:4000 (5%skimmed milk in TBST), 1h at RT
None
ECL detection in X-Ray film
This Santa Cruz antibody worked extremely well and got a dark, specific band of processed ADAM10 at 80kDa. However, there was no effect of treatment on ADAM10 expression. Santa Cruz ADAM10 antibody (sc-28358) is a very specific and strong antibody as it complies with the claims of datasheet.
Good value for your money and easy to use
Cheap in cost, best specificity and easy to detect
Some non-specific bands with light signal were detected below 50kDa
Excellent antibody