Western Blot Stripping Buffer Works Great and Can Re-Use

University of Nebraska Medical Center
Pharmacology and experimental neuroscience
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

Restore Western Blot Stripping Buffer

Catalog Number:

21059

For Western blot analysis, we strip the membranes so we can identify two proteins that are at very similar molecular weights.

Experimental Design and Results Summary

Application

Western blot

Starting Material

Protein

Protocol Overview

After membrane has been imaged with the first antibody, place the membrane in stripping buffer for 15 minutes and proceed with incubation in the secondary antibody you wish to use

Tips

You can re-use the stripping buffer

Results Summary

Signal from the first antibody is completely gone after stripping so it is easy to use the same membrane for multiple antibodies

Additional Notes

None

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Summary

The Good

Easy and can re-use

The Bad

None

The Bottom Line

Works great and is straightforward.

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