Excellent Kit to Determine IL-23 Levels in Serum

University of California
Internal Medicine
Assistant Project Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Mouse IL-23 (p19/p40) ELISA MAX™ Deluxe

Catalog Number:

433704

We use this kit on a regular basis to determine the levels of IL-23 in mice injected with various types of minicircle DNA. Because the sample size is large and we were aiming for a cheap kit that would determine the levels with high sensitivity, we decided to try this kit. BioLegend's kit is very simple to use and determines serum levels with great sensitivity and specificity. We have never had any problems with it with regards to background etc.

Experimental Design and Results Summary

Application

Determine the serum levels of IL-23 in mice injected with minicircle DNA.

Starting Material

None

Protocol Overview

Kit was used as per the BioLegend protocol. Serum quantity used varied between 1:5 dilution to 1:10

Tips

Not really. Background is nearly zero if instructions are followed properly

Results Summary

Generates good standard curve and concentrations as low as 20pg/ml were easily determined

Additional Notes

None

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Summary

The Good

Ease of use. Finishes within a day

The Bad

None

The Bottom Line

Excellent. Prefer this over other products available in the market. It's cheap and works best.

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