Abcam
Anti-Asporin Antibody
ab31303
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Browse Similar Products: ASPN Antibodies
Our longstanding goal is to understand the role of cancer-causing proteins and to elucidate the mechanism of tumorigenesis. Here we test the detection of asporin protein in different osteosarcoma cell lines using Western blotting and from the data it can be seen that the cells do express asporin, however given the polyclonal nature of the antibody, there seems to be other bands that may be non-specific. Overall this is a good antibody to detect using Western blotting, however the specificity needs to be further tested.
Western Blot
Human osteosarcoma cell lines
1:1000 in 5% BSA
5% BSA
1:1000 HRP
NA
Western blotting
Bands observed at ~43kDa, however other band at 29-30 kDa is non-specific.
None
Works well
Polyclonal
Can be used to detect ASPN