eBioscience
Anti-Mouse TCR beta PE
12-5961-82
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The goal of this experiment was to clearly distinguish T cells in the peripheral blood of female C57Bl/6 mice for subsequent phenotyping. I selected this product because TCRb is a T cell-specific surface marker.
Flow Cytometry
Peripheral blood leukocytes
Stained cells in 100 uL cold PBS containing Live/Dead and TruStain Fc block (2 ug/mL) on ice for 15 minutes.
TruStain Fc block (2 ug/mL)
Added 100 uL cold PBS containing isotype control (4 ug/mL) or TCRb PE (4 ug/mL) and incubated on ice for 15 minutes.
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Flow Cytometer
TCRb+ peripheral blood leukocytes were clearly distinguished from TCRb- peripheral blood leukocytes.
None
Cheap and clearly separates TCRb+ cells
Really the ideal antibody to identify TCRb+ cells, both for simple enumeration and subsequent phenotyping.