Great CD141 Antibody

Department of Hematology/Oncology
MSSM
Associate Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD141 VioBlue

Catalog Number:

130-097-325

The general aim of my research is identifying different markers for human dendritic cells. I chose this product because I find Miltenyi's products work very well for the BDCA line.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood DCs, differentiated DC cultures

Primary Incubation

1:100 dilution, 30 minutes at 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSR Fortessa

Results Summary

CD141 DCs were clearly identified using CD141 VioBlue and Clec9a PE

Additional Notes

None

Related Categories

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Summary

The Good

Miltenyi lot to lot works very well

The Bad

High volume of ul per sample

The Bottom Line

This is a very good color and easy way to identify CD141 DCs

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