TLR2 Staining of DCs

April 13, 2016

BlueskyReddit
Immunology
University of Rochester
Graduate Student

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Company:

BioLegend

Product Name:

TLR2 anti-mouse AlexaFluor647

Catalog Number:

121810

This antibody was used to assess TLR2 expression levels on naïve DCs in the spleen of BALB/c mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Spleen cells from BALB/c mice

Primary Incubation

30 min. Used manufacturer's suggested concentration.

Blocking Agent

FcBlock 1:100

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fortessa

Results Summary

TLR2 staining is very low on mouse DCs compared to isotype.

Additional Notes

None

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Summary

The Good

Ease of use

The Bad

Not bright enough

The Bottom Line

Ok antibody, maybe there is a better clone or fluorochrome.

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