ICAM-1 Expression in Lung Microvessels

Physiology
The University of Tennessee Health Science Center
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Company:

Santa Cruz Biotechnology

Product Name:

ICAM-1(1A29)

Catalog Number:

SC-19584

This was used to measure ICAM-1 expression in lung microvessels treated with LPS with different time points. (Refer to article in Microvasc Res. 2012 May;83(3):323-31. Real-time imaging reveals endothelium-mediated leukocyte retention in LPS-treated lung microvessels. Kandasamy K, Sahu G, Parthasarathi K.)

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Isolated blood perfused rat lung

Primary Incubation

30 min

Blocking Agent

5% FBS

Secondary Incubation

30 min

Tertiary Incubation

NA

Detection

Confocal microscopy

Results Summary

Please do refer to the article by Kandasamy K, Sahu G, Parthasarathi K. Real-time imaging reveals endothelium-mediated leukocyte retention in LPS-treated lung microvessels. Microvasc Res. 2012 May;83(3):323-31.

Additional Notes

None

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Summary

The Good

Ease of use

The Bad

None

The Bottom Line

Great for In situ immunofluorescence imaging

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