Nice Anti-TPX2 Antibody

April 08, 2016

BlueskyReddit
Pathology
University of Kansas Medical Center
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-TPX2 [clone 18D5-1]

Catalog Number:

ab32795

The focus of this work was to characterize expression of mitosis-related proteins in HeLa cells. This is a monoclonal antibody from Abcam, that has been refined to ensure high specificity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Ovarian Cancer Cell Lines

Primary Incubation

1:1000 in 5% milk in TBS-T; incubated overnight at 4 degrees C on a rocker

Blocking Agent

5% milk in TBS-T

Secondary Incubation

1:2500 anti-mouse HRP; 30 minutes at room temp on a rocker

Tertiary Incubation

NA

Detection

HRP Chemiluminescence

Results Summary

Exposure of the blot revealed a single, clean band at approximately 92kDa. That size is consistent with the known size of TPX2 in humans. There was a second larger band at approximately 97 kDa, which may be a posttranvslationally modified form of TPX2.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good antibody with a relatively clean band(s) at the correct predicted molecular weight.

The Bad

The antibody is a bit expensive, and there is a second band whose identity is unclear.

The Bottom Line

Pretty good antibody, will continue to use in the future.

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