Invitrogen
MegaX DH10B™ T1R Electrocomp Cells
C640003
Info: View Product View Product Specs
We are interested in making a large volume of lenti vector plasmid for genomics study. The MegaX 10B competent cells were transformed with ligation mixture contain lenti vector with insert. This competent cells were selected for this high transformation efficiency.
Library preparation
Ligation mixture
We used 2ul of ligation mixture into 22ul of electro competent cells with 2Kv, 200 ohm condition and typically get 4.5-4.7ms pulse.
Purify the ligation mixture before electroporation
We got high transformants, however with lentiviral vector we observed recombined plasmids if grown more than 14hr at 37 celsius.
None
High transformation efficiency
Observed unwanted recombinant in lenti vector with large volume.
Recommended for high efficiency transformation for library making.
Product Name: MegaX DH10B T1R Electrocomp CellsSupplier Page