Mouse IL-1 beta/IL-1F2 DuoSet ELISA

University of California, San Diego
Department of Pediatrics
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Mouse IL-1 beta/IL-1F2 DuoSet ELISA

Catalog Number:

DY401-05

This is a reliable ELISA, easy to use, and consistent between runs. It was used with murine lung homogenate.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Murine lung homogenate supernatant

Protocol Overview

Lungs from Mtb infected mice were homogenized in 5mL PBS-T. Homogenate was aliquoted and spun down at max speed, and the supernatant was filtered through a 0.2um PVDF filter. Sample was diluted 1:3 in the appropriate buffer as per product guidelines.

Tips

Make sure samples are within the standard curve

Results Summary

Great, reliable, and sensitive

Additional Notes

None

Image Gallery

Summary

The Good

Consistent results and easy to use

The Bad

None

The Bottom Line

Would use again

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