Review of the Anti-GRK5 Antibody (cat#PB9708)

April 04, 2016

Emergency Medicine
Thomas Jefferson University
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Bosterbio

Product Name:

Anti-GRK5 Picoband™ Antibody

Catalog Number:

PB9708

After using this antibody for western blot to analyze rat endothelial cells' protein, I found PB9708 Anti-GRK5 antibody worked very well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat endothelial cells

Primary Incubation

Anti GRK5 (PB 9708) at 1 ug/ml,4˚C overnight

Blocking Agent

5%(w/v) milk in TBST

Secondary Incubation

Anti-rabbit IgG(cell signaling,#7074) at 1:1000,2h, room temp

Tertiary Incubation

NA

Detection

Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096)

Results Summary

The blot of 68 Kda can be seen, however, we can see another 3 blots at least. In a word, Anti GRK5 (PB 9708) is useful and good.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

The blot of 68 Kda can be detected.

The Bad

There are some nonspecific blots.

The Bottom Line

Anti GRK5 (PB 9708) is useful, good.

Share your experience with other scientists. Write a Review! »

Join the discussion