Phosphorylation of p65 in Lung Microvessels

April 11, 2016

Physiology
The University of Tennessee Health Science Center
Instructor

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Quality of Results

Ease-of-Optimization

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Company:

Cell Signalling Technology

Product Name:

Phospho-NF-κB p65 (Ser536) Antibody

Catalog Number:

3031

This antibody was used for a published study, please refer to article: Lipopolysaccharide induces endoplasmic store Ca2+-dependent inflammatory responses in lung microvessels.Kandasamy K, Bezavada L, Escue RB, Parthasarathi K.PLoS One. 2013 May 10;8(5):e63465.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Isolated blood perfused rat lung

Primary Incubation

30 min

Blocking Agent

5% FBS

Secondary Incubation

30 min

Tertiary Incubation

NA

Detection

Confocal Microscopy

Results Summary

Please refer to article: Lipopolysaccharide induces endoplasmic store Ca2+-dependent inflammatory responses in lung microvessels.Kandasamy K, Bezavada L, Escue RB, Parthasarathi K.PLoS One. 2013 May 10;8(5):e63465.

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Best for immunofluorescence

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