Mouse Skin TGF beta 1

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Company:

Abcam

Product Name:

Anti-TGF beta 1 antibody [2Ar2]

Catalog Number:

ab64715

The aim is to detect TGF beta 1 expression in mouse skin. This is a mouse anti-mouse monoclonal antibody. The data sheet indicates that it reacts with mouse tissue. However, we did not get expected results after following the instructions to avoid high background issues.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse skin

Primary Incubation

1/400, 45min RT

Blocking Agent

Goat serum, Fc blocker, and unconjugated affinity purified F(ab) fragment anti-mouse IgG (H+L)

Secondary Incubation

Goat anti mouse Alexa Fluor 488

Tertiary Incubation

NA

Detection

Immunofluorescence

Results Summary

High background

Additional Notes

None

Related Categories

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Summary

The Good

None for mouse tissue

The Bad

High background

The Bottom Line

Not recommended for mouse tissue, instead please use ab92486 (rabbit anti- mouse).

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