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Date Published
Lenti Virus Purification in Short Time
(1)
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June 29, 2016
We use routinely in our lab to purify and concentrate lenti virus using this kit. The procedure is very simple and takes 30-40 min per ...
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Analyzing Efficacy of Small Molecule Blocker of TNF-a in Sorted CD4 T cells by using PE Conjugated anti-TNF-a Antibody and Flow Cytometry
(1)
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BioLegend
June 29, 2016
Small molecule TNF-a inhibitors have been used for blocking TNF-a pathways and anti-TNF-a drugs are now effectively used for immune ...
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CD31 gives strong endothelial cell staining
(1)
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June 29, 2016
We used this antibody to identify endothelial cells, which are CD45 negative and CD31 positive. Our research investigates the ability of ...
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Consistent CD11b staining
(1)
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June 28, 2016
We used this antibody to identify CD11b+ cells to help distinguish macrophages, dendritic cells, and other populations of APC. Our research...
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IRF5 Protein Levels in Human Sera
(1)
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CUSABIO Technology LLC
June 28, 2016
The commercially available kit was used for the quantification of human IRF5 protein levels. The instructions and protocols were easy to ...
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Poor Tiam1 Antibody
(1)
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Santa Cruz Biotechnology, Inc.
June 28, 2016
Present study was conducted to test the role of specific treatment on Tiam1 expression in human endothelial cells. Growth arrested ...
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The Working Antibody for Versican
(1)
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BosterBio
June 28, 2016
Our aim is to find if the TGF-beta superfamily member, Nodal, induces the expression of versican components. Since versican has several ...
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Great Balance for Fast Measurements
(1)
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June 28, 2016
In our lab, we focus on the development of new agents for imaging cancer. To do this, we must measure out the necessary reagents for ...
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FITC Mouse Anti-Human CD16 Flow Cytometry Staining
(1)
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BD Biosciences
June 28, 2016
Human monocytes were isolated from peripheral blood and stained with anti-CD16 to assess the purity of the cells.
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BioLegend Human IFN Gamma
(1)
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BioLegend
June 28, 2016
Our lab focuses uses TCR gene modified T cells to be used to adoptive T cell transfer. We retrovirally transduce activated T cells with an ...
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cDNA synthesis kit
(1)
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Bio-Rad
June 28, 2016
Kit is used to make cDNA from RNA.
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Human Fibroblast Activation Protein alpha /FAP Biotinylated Antibody Flow Cytometry Staining Using PE-Cy7 Streptavidin
(1)
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R&D Systems, a Bio-Techne Brand
June 27, 2016
Used the antibody in combination with PECy7 conjugated streptavidin to detect FAP expression on human synovial cells yielded by enzymatic ...
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Best NFkB Antibody from R&D Systems
(1)
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R&D Systems, a Bio-Techne Brand
June 27, 2016
Mouse pancreatic endothelial cells were tested for time course effect of growth factor on NF?B levels. Proteins were extracted from non-...
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PCR Amplification of DNA Templates for Cloning
(1)
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New England Biolabs
June 27, 2016
I have used phusion high fidelity polymerase for amplification of DNA templates/genes needed for cloning. I got good quality amplification ...
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Human STAT1-alpha protein levels in sera
(1)
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CUSABIO Technology LLC
June 27, 2016
The commercially available ELISA kit was used for the quantification of human STAT1-alpha levels in the sera of both patients and healthy ...
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Quantum Prep® Plasmid Miniprep Kit
(1)
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Bio-Rad
June 27, 2016
Bio-Rad's miniprep kit is similar to the standard prep protocols, but differs in one key manipulation: the user adds the plasmid DNA binding...
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Great Centromere Antibody
(1)
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June 27, 2016
ACA is an awesome centromere antibody to use, it's a human antibody, so it can be used with all other antibodies. It is a composite of cenpA...
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a-Synuclein
(1)
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BD Biosciences
June 27, 2016
We were evaluating if intra-cerebral 6-OHDA injection could modify a-synuclein expression in an animal model of Parkinson's Disease.
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I-Ad for MHC II staining in BALB/c mice
(1)
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June 27, 2016
We used this antibody to measure I-Ad levels from BALB/c or BALB/c x B6 mice. This antibody was particularly useful when we needed to ...
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Poor Fluorochrome for CD11c
(1)
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BD Biosciences
June 24, 2016
The purpose was a purity check of the CD11c+ cell fraction after MACs cell separation.
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V450 Mouse IgG1, kappa Isotype Control Clone MOPC-21 Flow Cytometry Staining
(1)
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June 24, 2016
Antibody was used to control for non-specific/Fc Receptor binding of human synovial cells analysed by flow cytometry.
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Great Epithelial Marker
(1)
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BioLegend
June 24, 2016
Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with ...
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Effectiveness of rabbit anti-14-3-3 eta (AHP1046, AbD Serotec) to detect the protein isoform in mouse oocytes and eggs
(1)
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Bio-Rad
June 24, 2016
The 14-3-3 (YWHA) proteins are central mediators in various cellular signaling pathways regulating development and growth, including cell ...
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Excellent Antibody to Detect IL-33 in Human Cells
(1)
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R&D Systems, a Bio-Techne Brand
June 24, 2016
Aim of the study was to test the role of specific growth factor on IL-33 expression in human smooth muscle cell. Proteins from each ...
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CHOP Antibody
(1)
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Thermo Fisher Scientific
June 24, 2016
To asses ER-stress, astrocytes were treated for 24 hs, with increasing concentrations of tunicamycin. Total proteins were analyzed by ...
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Quick, Good-quality Sequencing
(1)
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Retrogen
June 24, 2016
Retrogen provides daily sample pick-up and next-day sequencing with consistently good sequence quality or complimentary repeat sequencing if...
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CD11c FITC - Reliable Staining
(1)
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June 24, 2016
We used this antibody to identify dendritic cell and macrophage subsets. Our research investigated antigen processing and presentation ...
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A Good Antibody for Detecting Human NCR1 (Nkp46, CD335)
(1)
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June 23, 2016
NCR1 (also known as Nkp46 or CD335) is a useful marker for identifying NK cells in the peripheral blood. Our lab routinely performs FACS ...
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Mouse IgG2a, monoclonal (FITC) - Isotype control flow cytometry
(1)
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Abcam
June 23, 2016
The isotype control was used to control for non-specific/Fc receptor binding in human synovial cells
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Seahorse Plate
(1)
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Agilent Technologies
June 23, 2016
The research aim of this current study is to test how lipid regulates cell metabolism. Specifically, in this experiment, we aim to study how...
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Not a Good Leukocyte Marker
(1)
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BioLegend
June 23, 2016
Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with ...
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YA-E Antibody
(1)
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June 23, 2016
This antibody detects the Ea52-68 peptide bound to I-Ab MHC II molecules. This can be found in B6 x BALB/c mice, but not in either B6 or ...
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Great Block for Flow Cytometry
(1)
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BioLegend
June 23, 2016
I'm using flow cytometry to characterize cell populations in the lung. I chose this product because my CD16/32 block was getting old and ...
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Detection of NALP3 in human endothelial cell using Adipogen life sciences antibody
(1)
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June 23, 2016
Time course effect of specific growth factor on NALP3 expression levels were tested in human endothelial cells. Growth factor treated or ...
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Centrifuge
(1)
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June 23, 2016
Refrigerated Centrifuges are useful for centrifugation of temperature sensitive materials. This is basic instrument in research labs, ...
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Inconsistent Results with BCA Protein Assay Kit
(1)
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June 22, 2016
I am currently studying the impact that BST-2 has on various enveloped viruses and one nonenveloped viruses via si RNA transfection to ...
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Good anti-pGSK3 beta Antibody
(1)
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Abcam
June 22, 2016
Human aortic endothelial cells were subjected to growth factor treatment for various time points and tested for its effect on phospho GDK3 ...
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Anti-CD31 Antibody [TP1/15] (FITC) Flow Cytometry
(1)
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Abcam
June 22, 2016
Anti-CD31 was used to stain human synovial cells to identify the presence of endothelial cells
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Excellent for Western Blotting
(1)
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Thermo Fisher Scientific
June 22, 2016
We wanted to detect glutathione peroxidase 1 (GPX1) in HK2 cells (human kidney cell line). We selected this antibody because it was the ...
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Single Cell isolation from Adult Mouse Brain
(1)
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Miltenyi Biotec
June 22, 2016
We use in our lab this Kit combined with MACS gentle dissociator to dissociate the adult brain from the mouse. The protocol is highly ...
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Live Dead Aqua is a Good Fixable Viability Stain
(1)
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June 22, 2016
We used this antibody to distinguish between live and dead cells when we needed to be able to fix the cells prior to running them on the ...
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This Isotype Control May Need Its Own Control
(1)
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June 22, 2016
I am analyzing immune cell populations in the murine lung and how they change following viral infection. I needed an isotype control for ...
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Epithelial Progenitor Marker
(1)
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June 22, 2016
Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with ...
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Great Antibody
(1)
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BioLegend
June 21, 2016
I used this antibody in my flow cytometry panel in hopes of defining my CD4 T cell population. This antibody is a little nerve-racking ...
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Good MHC II staining
(1)
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June 21, 2016
We used this antibody to measure the level of MHC II on different populations of murine lymphocytes and splenocytes. Our research was ...
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Good Quality, Great Price, Accurate IL-8 Readings
(1)
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BioLegend
June 21, 2016
Measurement of IL-8 in media supernatant is a commonly used technique in immunology labs. It is secreted by a wide variety of cells, and can...
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Excellent Ga(q) Antibody from Cell Signaling Technology
(1)
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Cell Signaling Technology
June 21, 2016
Aim of the study was to test the effect of Ga(q) specific siRNA on Ga(q) expression levels in human smooth muscle cells. Cells which were ...
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Good Human IL-17 A Antibody
(1)
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BioLegend
June 21, 2016
Human PBMC were stimulated for 6 hrs with PMA/Ionomycin in the presence of GolgiStop. Cells were surface stained first with Pacific blue ...
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Concentrator
(1)
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SP Scientific
June 21, 2016
This instrument is awesome. it has been designed specifically for solvent removal in various areas of scientific research. It can also use ...
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PE Mouse Anti-Human CD14 Clone MfP9 Flow Cytometry Staining
(1)
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BD Biosciences
June 21, 2016
The anti-human CD14 antibody was used to stain monocytes isolated from human leukaphereisis products to assess for the purity of the cells.
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