Live Dead Aqua is a Good Fixable Viability Stain

June 22, 2016

Immunology
University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Live/dead aqua

Catalog Number:

L34957

We used this antibody to distinguish between live and dead cells when we needed to be able to fix the cells prior to running them on the flow cytometer. Our research investigates antigen processing and presentation in a variety of murine cell types.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:200 dilution for 10 minutes at room temperature in PBS

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This provides acceptable delineation between live and dead cells. The staining is not as bright as DAPI would be, but it is perfectly adequate for most uses. One disadvantage is that staining must be done in PBS, which can be problematic for very fragile cell types.

Additional Notes

None

Related Categories

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Summary

The Good

Sufficient delineation between live and dead cells

The Bad

Requires staining in PBS at room temperature

The Bottom Line

Good option when you need to be able to fix the cells. Also works with subsequent intracellular permeabilization protocols.

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