Bio-Rad
Quantum Prep® Plasmid Miniprep Kit
7326100
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Bio-Rad's miniprep kit is similar to the standard prep protocols, but differs in one key manipulation: the user adds the plasmid DNA binding matrix to the plasmid-containing solution, instead of simply adding the latter to a spin column. The kit is easy to use, rapid, and delivers a good yield of DNA.
Plasmid miniprep
Bacterial culture (3 ml)
Standard miniprep protocols involve having the user lyse the bacteria, and neutralize the lysis buffer, followed by adding the cleared lysate to a spin column. The Quantum Prep differs by having the user add the plasmid DNA binding matrix to the plasmid-containing solution, allowing binding, and then pelleting the matrix. The matrix is then washed once, and resuspended and added to a spin column, allowing for elution of the plasmid. In the first figure, we see the plasmid yield at about 6 -8 kb in size for 5/6 colonies. The second figure shows the five positive plasmids after digestion with EcoRI and EcoRV, which yielded a band of ~600 bp.Something worth noting. I had purchased this kit 10 years ago and forgot about it. I discovered it the other day, and amazingly, it still worked! That's saying something about Bio-Rad quality.Overall the kit is easy to use, rapid, and delivers a good yield of DNA.
I used TE heated to 70-80 degrees C to better elute the DNA.
Excellent yield of plasmid, which allowed for further downstream restriction enzyme digestion.
None
Rapid, easy to use, with good yield
List price is about $2/miniprep, which is somewhat higher than other kits.
Excellent kit.
Product Name: Quantum Prep® Plasmid Miniprep KitSupplier Page