Agilent Technologies
Seahorse XFe96 FluxPaks
102416-100
The research aim of this current study is to test how lipid regulates cell metabolism. Specifically, in this experiment, we aim to study how fatty acids regulate CD8 T cell mitochondrial oxidative phosphorylation. Briefly, T cells were seeded in the plate and loaded to the seahorse analyzer. Mitochondrial drugs were injected in the following order: oligomycin, FCCP, Etomoxir (an inhibitor of fatty acid transportation to mitochondria and Rotenone). Oxygen consumption rate (OCR) was monitored. Seahorse Bioscience (with Agilent Technologies) offers a very convenient tool to address this question, and that is why I choose this product for my experiment.
Be careful of the barcode of the plate.
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Easy to use.
A little expensive.
In general, the product fulfill my experimental purpose. Although it is a little expensive, it is easy to use and the experimental result is of some value for my project.