Great Block for Flow Cytometry

Biological Sciences
University of Idaho
Graduate student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

TruStainfcX (anti-mouse CD16/32)

Catalog Number:

101320

I'm using flow cytometry to characterize cell populations in the lung. I chose this product because my CD16/32 block was getting old and this was a recommended product.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lungs

Primary Incubation

2 uL per 10^6 cells, 15 minutes on ice

Blocking Agent

This is the blocking agent

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

It helped clear my results up and prevent most nonspecific binding. It allowed me to better characterize my cell populations and trust my other antibodies.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use.

The Bad

This has been a good product so far, no real drawbacks yet.

The Bottom Line

I will continue to use the product in my flow cytometry preparations. I also recommend it for others in my lab and school.

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