FITC Mouse Anti-Human CD16 Flow Cytometry Staining

June 28, 2016

Inflammation & Ageing
University of Birmingham
PhD Student

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Quality of Results

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Company:

BD Biosciences

Product Name:

FITC Mouse Anti-Human CD16 Clone 3G8 (RUO)

Catalog Number:

555406

Human monocytes were isolated from peripheral blood and stained with anti-CD16 to assess the purity of the cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human monocytes isolated from leukapheresis products.

Primary Incubation

30 minute incubation at 4oC. Antibody was used at a 1 in 10 dilution.

Blocking Agent

No specific blocking step. 2% FCS in staining buffer.

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR X-20 Fortessa. Analysis using FlowJo v10

Results Summary

Antibody works well to identify a clear CD16 positive population allowing for purity checks/subset analysis on isolated monocytes.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Nice bright antibody staining.

The Bad

Antibody has to be used at a low concentration but a large volume is supplied

The Bottom Line

A good antibody with very bright staining.

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