eBiosciences
Anti-Mouse Ea52-68 peptide bound to I-Ab Biotin (YA-E antibody)
13-5741-81
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This antibody detects the Ea52-68 peptide bound to I-Ab MHC II molecules. This can be found in B6 x BALB/c mice, but not in either B6 or BALB/c homozygous mice. We used this antibody to investigate the functionality of the antigen processing and presentation pathways in different murine cell populations.
Flow Cytometry
Murine lymphocytes
YAE biotin antibody at 1:200 for 30 minutes at 4 degC
2.4G2 Fc block
Strepavidin PE at 1:100 for 30 minutes at 4 degC
None
Canto II flow cytometery
This antibody provided reliable, clear staining. In the attached picture, the gray line represents the background staining in B cells from a B6 mouse. The red line shows YA-E staining in B cells from a B6 x BALB/c mouse. This was extracellular staining.
Reliable, strong staining
Biotin antibodies have the potential for increased background staining, but that was not seen here
Good strong & reliable staining.