a-Synuclein

Pathology
Federal University of Paraná
Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Purified Mouse Anti-a-Synuclein

Catalog Number:

610787

We were evaluating if intra-cerebral 6-OHDA injection could modify a-synuclein expression in an animal model of Parkinson's Disease.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Brain protein extract

Primary Incubation

1:3000, Over night, 4˚C, incubated in 5% slime milk in TBS-T

Blocking Agent

5% slime milk in TBS-T

Secondary Incubation

1:4000, 1 hour, room temperature, incubated in 5% slime milk in TBS-T

Tertiary Incubation

Not necessary

Detection

Chemiluminescent detection

Results Summary

The antibody works really good, it shows a only one beautiful band at 18-19 KDa.

Additional Notes

For this result I loaded 5ug of proteins

Related Categories

Image Gallery

Summary

The Good

Excellent result

The Bad

Nothing

The Bottom Line

Never disappoints me

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