Abcam
Mouse IgG2a, monoclonal (FITC) - Isotype control
ab81197
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The isotype control was used to control for non-specific/Fc receptor binding in human synovial cells
Flow Cytometry
Human synovial cells
30 minute incubation at 4oC. Live/dead stain.
No specific blocking step. 2% FCS in staining buffer.
30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 3ug/ml.
None
Mo Flow Astrios EQ 488 excitation 530/40 bandpass filter for emission
Antibody worked as expected of an isotype control showing no positive cells and little to no non-specific/Fc receptor binding
Antibody worked as expected.
No particular bad points
A decent isotype control that works as one would expect