Thermo Scientific
CHOP Antibody (9C8)
MA1-250
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Browse Similar Products: CHOP Western Blot Antibodies
To asses ER-stress, astrocytes were treated for 24 hs, with increasing concentrations of tunicamycin. Total proteins were analyzed by western blot against CHOP.
Western Blot
Protein extract of cultured astrocytes
1:1000, Over night, 4˚C, incubated in 5% slime milk in TBS-T
5% slime milk in TBS-T
1:4000, 1 hour, room temperature, incubated in 5% slime milk in TBS-T
Not necessary
Chemiluminescent detection
Western blot detected an approximately 30 KDa protein, representing CHOP
None
It works fine
It was a little difficult to standardize
It's not the best, but I would recommend it for western blot (never tested for IHQ/ICC)