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Date Published
Detecting TGFß1 in Human Sarcoma
(1)
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Santa Cruz Biotechnology, Inc.
September 05, 2014
The TGF family of proteins are implicated in many tumor processes, such as development, growth, migration, and metastasis. A good antibody ...
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Are your T cells activated?
(1)
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BioLegend
September 05, 2014
The stimulation of T cells is carried out by different methods. In order to check if your stimulation worked or not, there are many factors ...
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Muscle Actin Identification by IHC
(1)
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September 05, 2014
We use this antibody for some tumor types classifications. It can recognizes specific Actin present in skeletal muscle, not in smooth muscle...
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Western Blotting - CLK2 Antibody in Human Endothelial Cells
(1)
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Aviva Systems Biology
September 05, 2014
Clk2 protein expression was characterized via western blot using the CLK2 Antibody (1:1000).
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Determination of the IgG Subclass of a Human Autoantibody
(1)
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Abcam
September 02, 2014
Our laboratory is interested in an autoantibody that we identified several years ago in cancer patients. The presence of this autoantibody ...
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Great anti-FLAG Monoclonal Antibody
(1)
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September 02, 2014
A FLAG tagged construct was overexpressed in HEK293 cells and WB was utilized to track the expression level of the protein.
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Norgen Urine Total RNA Purification Kit
(1)
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Norgen Biotek Corporation
September 02, 2014
I am interested in the expression of genes in the urine of transplant, in order to identify biomarkers for a disease called graft versus ...
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DNA and Protein Visualization
(1)
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ProteinSimple, a Bio-Techne Brand
September 01, 2014
This equipment was installed as shared facility 3 years back at Ohio Sate University in building named Tzagournis. Our lab work involves ...
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PCR Purification via MinElute PCR Purification Kit
(1)
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September 01, 2014
We study the various mechanisms of gene silencing responsible for disease resistance in poultry. We specifically study various target genes ...
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Kit for isolation of DNA, total RNA and protein
(1)
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August 21, 2014
This kit was used to collect DNA, RNA, and protein for PCR, qPCR, and Western blotting for a circadian rhythms experiment. We thought that ...
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Use of Tendem Dye for Multicolor Flow Cytometry
(1)
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BioLegend
August 15, 2014
The aim of my study was to investigate CD4+ T cell proliferation. I harvest mouse splenocytes and use following antibodies from Biolegend ...
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Great Transfer Apparatus
(1)
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Bio-Rad
August 14, 2014
I use this apparatus to transfer proteins from SDS-PAGE gels to PVDF membranes for immunoblotting.
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Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb antibody (for Western Blot) from Cell Signaling Techonology
(1)
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Cell Signaling Technology
August 08, 2014
This antibody was used in a study that investigated the role of Phospho-p70 S6 Kinase (Thr389) in WT and transfected cardiomyocytes. Several...
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qRT-PCR Primers
(1)
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Integrated DNA Technologies
August 08, 2014
It is a great pleasure to write review on Integrated DNA technologies which provides qRT-PCR primers. We were screening expression of 500 ...
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Erba Manheim SGPT Assay Kit
(1)
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August 07, 2014
As part of my work, I was in a search of a highly specific and sensitive protocol for estimation of SGPT as I am working on phytochemicals ...
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Application of PE Conjugated Anti Human MDR1 Ab
(1)
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BioLegend
August 07, 2014
We are trying to look at the expression of MDR1 in different cell lines by FACS. This Ab works great, as it can be directly used to stain ...
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Erba Mannheim Calcium Assay Kit
(1)
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August 07, 2014
I am working on phytochemicals in the area of renal research and I wanted to analyze the effect of phytochemicals on the extent of stone ...
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Erba Manheim Urea Assay Kit
(1)
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August 07, 2014
Urea is a key major end product of protein metabolism in humans. It constitutes the largest fraction of non-protein nitrogen component of ...
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Works for Most of Proteins
(1)
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Thermo Fisher Scientific
August 07, 2014
Gradient gels have several advantages for protein separation regarding the sharpness of bands and allowing to separate wider range of ...
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Simple Cloning for Protein Expression
(1)
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August 07, 2014
I've used DNA 2.0 to synthesize codon optimized genes for protein expression for over a dozen projects. Their service is very reliable. ...
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Erba Manheim Creatinine Assay Kit
(1)
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August 07, 2014
Creatinine is a key waste product in muscle from high energy compound, creatine phosphate. The amount of creatinine produced is highly ...
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The Usage of PP2Ac ab
(1)
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MilliporeSigma
August 06, 2014
To detect the protein abundance of PP2Ac in arabidopsis with this PP2Ac antibody. This antibody uses the most conserved sequence of PP2Ac ...
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Great Kit for RNA Extraction
(1)
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QIAGEN - Sample to Insight
August 06, 2014
Extraction of total RNA from brain tissue for use in qPCR experiments. Selected this product in order to purify RNA extracted using phenol:...
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NFkB - p65 Detection by WB
(1)
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Santa Cruz Biotechnology, Inc.
August 06, 2014
NfKB pathway is one most important transcriptional regulator in many cellular and physiological processes. Destabilization of NfKB protein ...
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Intracellular IFN-g Staining for Mouse Cells by Flow Cytometry
(1)
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BioLegend
August 06, 2014
Mouse splenocytes were activated by anti-CD3/anti-CD28 for 96 hours, in polarizing conditions towards Tc1/Th1 or Tc17/Th17, followed by ...
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EMT Analysis: Lef1 Detection by WB
(1)
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Cell Signaling Technology
August 06, 2014
Epithelial to Mesenchimal Transcription (EMT) is a cellular process in which cells transform their phenotype into a more fibroblast-like one...
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An Antibody that Serves the Purpose, Wonderfully
(1)
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Novus Biologicals, a Bio-Techne Brand
July 29, 2014
I am wanted to check the level of MLL1 in HL-60 and HEk-293T cells before I could silence the gene and study its effects.
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Very Good Antibody for Actin Western Blot
(1)
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Novus Biologicals, a Bio-Techne Brand
July 28, 2014
I use this antibody as a loading control for the western blots.
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Mfn-1 in Immuno-histochemistry
(1)
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Novus Biologicals, a Bio-Techne Brand
July 25, 2014
This product was chosen because adequate information was available on the product website to aid with the decision.
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Efficient Silica-Based Genomic DNA Extraction from Blood and Tissues
(1)
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QIAGEN - Sample to Insight
July 24, 2014
I use the kit routinely to extract gDNA from turkey liver or blood samples for SNP genotyping.
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CD9: Just Great
(1)
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BioLegend
July 23, 2014
We selected CD9 Antibody from Biolegend specifically because CD9 is expressed in some myeloid progenitor cells and can be found in bone ...
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CD16/32: Good Reagent
(1)
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BioLegend
July 22, 2014
We selected CD16/32 Antibody from Biolegend specifically because CD16/32 is expressed in myeloid progenitor cells and can be found in bone ...
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CD41: Very Useful Reagent
(1)
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BioLegend
July 21, 2014
We selected CD41 Antibody from Biolegend specifically because CD41 is expressed in some progenitor cells and can be found in bone marrow.
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CD48: Dim Fluorochrome
(1)
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BioLegend
July 18, 2014
We selected CD48 Antibody from Biolegend specifically because CD48 is expressed in myeloid cells and can be found in bone marrow.
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CD115: Good and Useful Reagent
(1)
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BioLegend
July 17, 2014
We selected CD115 Antibody from Biolegend specifically because CD115 is expressed in bone marrow progenitor cells and can be found in adult ...
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CD117 (c-Kit): Just Great
(1)
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BioLegend
July 16, 2014
We selected CD117 Antibody from Biolegend specifically because CD117 is expressed in progenitor cells and can be found in adult bone marrow.
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Exosome Isolation Reagent from Life Technologies
(1)
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Thermo Fisher Scientific
July 15, 2014
I have used the Life technologies Exosome Isolation reagent to isolate exosomes from human serum.
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Chromatin Immuno-precipitation Made Easy
(1)
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July 14, 2014
Our research lab is a HIV lab and we study how HIV affects the immune system.
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Effect of Hyperoxia on TICAM1 Expression
(1)
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BioLegend
July 11, 2014
TICAM-1 negatively regulates TLR signalling, so we wanted to check whether hyperoxia somehow affects TICAM-1 expression and disrupts TLR ...
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Difficult to Optimize but Good Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 10, 2014
I wanted to use this antibody as a marker for aging caused H-Ras induced senescent in BJ cells
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Ki67 Immuno-fluorescence Staining
(1)
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BioLegend
July 09, 2014
The antibody was used to examine tumor cell proliferation in the context of a spontaneous intestinal tumor model (the ApcMin mouse).
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Good Antibody for Axon Identification
(1)
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Santa Cruz Biotechnology, Inc.
July 08, 2014
The described antibody was used to mark the axon initial segment in rat hippocampal neurons. This marker was chosen because it makes early ...
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S100A4 Detected by IHC
(1)
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MilliporeSigma
July 07, 2014
We use this marker to detect those active fibroblasts in tumoral samples to analyze patient or mice tumoral samples.
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Good Reagent: CD127 Antibody
(1)
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July 03, 2014
We selected CD127 Antibody from BD specifically because CD127 is expressed in common lymphoid progenitor cells and can be found in adult ...
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Excellent Product to Test Picogram Levels of IL-27 Heterodimer in Mouse Serum
(1)
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BioLegend
July 01, 2014
We overexpress IL-27 in the mice for determining its role in osteoclastogenesis. Mice serum are collected to check for the circulating IL-27...
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Lots of DNA in Minimum Time and Effort
(1)
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QIAGEN - Sample to Insight
June 27, 2014
Working in the field of basic biology, everyone needs to use plasmids for cloning followed by transfections. I use this kit to purify ...
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Great Ki67 Antibody at Optimal Cost
(1)
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Abcam
June 26, 2014
The main aim of our research is to see the proliferation of cancer cells in 3D spheroids. Specific goal: Identify the cancer cells ...
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Cyclin T1 Immunofluorescence
(1)
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Santa Cruz Biotechnology, Inc.
June 26, 2014
Works great, low background in non-expressing monocytes, good signal in differentiated macrophages.
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Strong Signal: Anti-CTCF Immunofluorescence
(1)
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MilliporeSigma
June 25, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of CTCF in human cells by indirect ...
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Excellent Cheap Way to Make Competent E.coli
(1)
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June 25, 2014
My research focuses on T cell immunotherapy using lentiviral vectors. For this, i need to keep cloning various inserts into lentiviral ...
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