R&D Systems
Human NKG2C PerCP MAb (Clone 134591)
FAB138C
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We wanted to use the antibody to identify the memory NK cells in CMV seropositive individuals, in conjunction with other markers such as CD57. To fit into a large panel of markers we wanted to use this antibody conjugated with PerCP.
Flow Cytometry
Human PBMCs
Used 1 in 50, stained for 25min at 4 degrees.
Did not use
NA
Flow cytometer
The antibody did stain the NK cells when the cells were stained on the surface. However following fixation and permeabilisation, the staining was lost.
None
The antibody stains correctly on the surface.
The staining is not bright, and is disrupted by fixing and permeabilisation.
The antibody works but is not the best - other fluorochromes work better for this antibody.