PE anti-mouse IL-22 Antibody for ICS

Cancer Biology
Scripps research center in FL
Research Associate

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Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse IL-22 Antibody

Catalog Number:

516404

Info: View Product View Product Specs

Browse Similar Products: IL-22 Antibodies

We transfer wild type CD4 T cells to Rag-/- mice to establish a Colitis model and then look at the phenotype of these transferred CD4 T cells via intracellular cytokine staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:10 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

After transplantation, mouse spleen was collected. IL-22 expression was gated in CD45+CD3+CD4+ T cells.

Additional Notes

None

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Summary

The Good

Easy application

The Bad

Needs high concentration

The Bottom Line

Good antibody for FACS

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