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Date Published
Good early stem cell marker
(1)
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MilliporeSigma
December 05, 2014
These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the ...
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Excellent ab for CD4 population staining
(1)
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BioLegend
December 04, 2014
This product was used for experiments running phenotypic detection of CD4 T cells and their roles in a mouse colitis model. This mouse CD4 ...
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Very reliable alpha-tubulin antibody
(1)
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Abcam
December 04, 2014
Tubulin is used as a loading control at 50KDa for antibodies that are lower than 40KDa. Most other loading markers fall under 40Kda which ...
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HSP60 antibody
(1)
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December 04, 2014
HSP60 has been used as a loading control for mitochondrial protein. It showed the same level of actin as another loading control for ...
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MIF quantitative ELISA
(1)
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BioLegend
December 03, 2014
MIF levels have been shown to be high in patients with acute dengue. Therefore, we set out to validate if this can be used as a biomarker to...
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Excellent Ab for IL-17 intracellular staining
(1)
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BioLegend
December 02, 2014
This Ab was used to detect intra-cellular staining of IL17 from mouse CD4 T cell differentiated pathogenic Th17 cells in vitro.
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Excellent ab for CD44 memory T cell population staining
(1)
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BioLegend
December 02, 2014
This product was used for experiments running phenotypic detection of CD4 T cells and their roles in a mouse colitis model. This ab can be ...
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FFPE Immunohistochemistry of C5b-9
(1)
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Abcam
December 02, 2014
Activation of the complement system ultimately leads to the formation of the membrane attack complex, or MAC. The MAC is composed of the ...
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An alternative anti-EpCAM antibody
(1)
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December 01, 2014
EpCAM is a marker used very often in research to detect epithelial cells. This is very important in mixed populations (e.g. tumors ...
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Imaging CD14 positive human monocytes in peripheral blood mononuclear cells (PBMC) using wide-field microscope
(1)
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Abcam
December 01, 2014
We isolated peripheral blood mononuclear cells from fresh human blood using Ficoll Paque Plus protocol and stained them with anti CD14-FITC ...
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Purification of PCR products for cloning
(1)
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Promega
December 01, 2014
I routinely cloning PCR products to various plasmids for expression and conditional knock down experiments. I need high purity PCR product ...
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Failed to improve GFP signal
(1)
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MilliporeSigma
November 26, 2014
Embryonic rat dorsal root ganglion neurons were infected with a GFP-tagged lentivirus in culture. The signal from the virus was present, ...
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Mitochondrial marker for WB- AIF antibody
(1)
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Santa Cruz Biotechnology, Inc.
November 26, 2014
HEK293 cells were fractionated into cytosol and heavy membrane fractions. AIF was utilized to track the purity of the fractionation as AIF...
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Loading Control: Tubulin (a must have for WB)
(1)
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November 26, 2014
Western blot detection usually requires the use of loading controls to interpret the results of experiments. Tubulin is ubiquitously ...
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Acetylated tubulin Ab
(1)
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November 25, 2014
I have used this on non-model organisms for staining neurons and worked great!
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S100b
(1)
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Abcam
November 25, 2014
These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the ...
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Itgav protein expression analysis via Western blotting
(1)
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Abcam
November 24, 2014
Itgav protein expression was characterized via Western blot using the Integrin alpha V antibody (1:500).
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Very potent antibody for pERK flow staining
(1)
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Cell Signaling Technology
November 24, 2014
Upon T-cell receptor activation, T cells turn on MAPK pathways including the ERK (p44/42) activation, which is critical for T cell ...
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VEGF protein expression - Western blotting
(1)
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November 24, 2014
VEGF protein expression was characterized via Western blotting using the VEGFA polyclonal antibody (1:1000) from Abnova
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Staining for Mfn 2 in neural stem cells
(1)
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Abcam
November 21, 2014
Anti Mfn-2 was used to detect effect of environmental toxicants on mitochondrial fusion in stem cells. This antibody worked well and the ...
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P38 protein expression was characterized via Western blotting using the Anti-p38 MAP Kinase (341-360) Rabbit pAb
(1)
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MilliporeSigma
November 21, 2014
P38 protein expression was characterized via Western blotting using the Anti-p38 MAP Kinase (341-360) Rabbit pAb (1:1000) in HL-1 cells
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Phospho-p38 - Western Blot
(1)
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Cell Signaling Technology
November 21, 2014
phospho- p38 protein expression was characterized via Western blotting using the Phospho-p38 MAPK (Thr180/Tyr182) Antibody from Cell ...
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Fluorescent Detection Reagent Eases LAMP Detection
(1)
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November 20, 2014
Our lab was conducting Loop-mediated isothermal amplification (LAMP) experiments using both SA scientific kits and bst 2.0 homemade ...
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Cell screening kit: Easy to use, great data
(1)
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BioLegend
November 20, 2014
I found the Biolegend LEGENDScreen incredibly easy to use and following the manufacturer's instructions produced great data to help me ...
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Beautiful neuronal staining
(1)
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Bio-Rad
November 19, 2014
These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the ...
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Nice Astrocyte Marker
(1)
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Abcam
November 19, 2014
These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the ...
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SC35 protein expression analysis via Western blotting
(1)
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Abcam
November 18, 2014
SC35 protein expression was characterized via western blot using the SC35 Antibody (1:1000)
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Lipid ELISA
(1)
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November 18, 2014
Our lab studies a specific subset of B cells. Our research focuses on determining the overall function of these B cells in both human and ...
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Anti HSP-10 staining on stem cells
(1)
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Santa Cruz Biotechnology, Inc.
November 17, 2014
The antibody was used to detect the effect of environmental toxicants on mitochondrial unfolded protein response. Specifically, I used this...
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HSP-60 antibody staining on stem cells
(1)
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November 17, 2014
The antibody was used to detect the effect of environmental toxicants on mitochondrial unfolded protein response. Specifically, this ...
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Phospho Akt protein expression analysis via Western blotting
(1)
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Cell Signaling Technology
November 14, 2014
Phospho Akt/PKB protein expression was characterized via Western blot using the Phospho-Akt (Ser473) Mouse mAb (1:1000).
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Excellent Ab for intracelluar staining of mouse IL-10
(1)
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BioLegend
November 14, 2014
We are looking at the intracellular staining of mouse IL-10 in mouse CD4 T cells from the spleen when stimulated by PMA, Inomycin and BFA. ...
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Akt/PKB protein expression analysis via Western blotting
(1)
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Cell Signaling Technology
November 13, 2014
Akt/PKB protein expression was analyzed via Western blotting with Akt Mouse mAb (1:1000) in murine cardiomyocytes
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ChIP grade HIF1-Alpha
(1)
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Thermo Fisher Scientific
November 13, 2014
HIF1-Alpha is a trancriptional factor which is expressed upon deprivation of oxygen concentration and regulates several downstream targets. ...
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Mouse specific cleaved PARP (cell death marker)
(1)
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November 12, 2014
Programed cell death (PCD) is a critical process that underlies normal tissues development and serves as a homeostatic mechanism to maintain...
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Cleaved caspase-3 Antibody for WB
(1)
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Cell Signaling Technology
November 12, 2014
Programed cell death (PCD) is a critical process that underlies normal tissue development and serves as a homeostatic mechanism to maintain ...
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Great Antibody for FLAG immunoprecipitations
(1)
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November 11, 2014
Our lab uses Anti-FLAG® M2 Magnetic Beads (produced in mouse, Sigma) for protein interaction co-immunoprecipitations. Invariably, some of ...
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Clear and Crisp DnaK Antibody
(1)
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Abcam
November 11, 2014
We generally use DnaK protein levels as a cytosolic control for western blotting. This allows us to determine if general protein levels are ...
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Western blotting via anti-Bcl-x
(1)
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November 10, 2014
Anti-Bcl-x protein expression was characterized via western blot by using purified rabbit Anti-Bcl-x (1:500)
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MCP-1 detection in A549 cells
(1)
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Santa Cruz Biotechnology, Inc.
November 10, 2014
MCP-1 protein expression was characterized via Western blot by using purified MCP-1 (C-17) Antibody
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GAPDH protein expression analysis via Western blotting
(1)
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MilliporeSigma
November 07, 2014
GAPDH protein expression was characterized via Western blot using the anti-GAPDH antibody (1:10000).
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Western blot via p44/42 MAPK (Erk1/2) Mouse mAb from Cell Signaling Technology
(1)
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Cell Signaling Technology
November 07, 2014
Erk1/2 protein expression was characterized via Western blot in HL-1 cells
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Excellent Ab for intracellular staining of mouse IFN-g
(1)
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BioLegend
November 06, 2014
We are looking at the intracellular staining of mouse IFN-gamma in CD4 memory T cells when stimulated by PMA, Ionomycin and BFA.
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CD3 Antibody works at high concentration
(1)
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BioLegend
November 06, 2014
We are trying to sort CD3 population in mouse spleen cells by Brilliant Violet 650™ anti-mouse CD3 Antibody.
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SuperBlock Really is Super
(1)
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November 05, 2014
I have been working with SuperBlock as a blocking buffer for the last three years and it has dramatically improved the quality of my western...
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QIAxcel for Saving Time
(1)
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QIAGEN - Sample to Insight
November 05, 2014
Our lab studies a specific subset of B cells. Our research focuses on determining the overall function of these B cells in both humans and ...
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A great DNA ladder
(1)
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November 04, 2014
We run a lot of 1-2% agarose gels to test the size of our sample DNA, especially PCR products. We have used several types of ladders in the ...
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Primer is superier to other version of ECL
(1)
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November 04, 2014
I was trying to get a Western blot done for tissue expression of the trans gene from transgenic mice we created. I was having trouble with ...
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PCR amplification using REDTaq® ReadyMix™ PCR Reaction Mix
(1)
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November 03, 2014
I have used the REDTaq ReadyMix PCR reaction mix to amplify mutation containing regions of DNA for downstream Sanger sequencing.
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Very good CD304 antibody
(1)
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BioLegend
November 03, 2014
We use this antibody to discriminate between thymus derived versus peripheral induced regulatory T cells in mouse spleen on a regular basis....
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