Cell Signaling Technology
Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
2211
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This antibody was used in a study that investigated the role of Phospho- S6 Ribosomal Protein (Ser235/236) in fibroblast cells from a neonatal human and a patient with de novo cardiovascular diseases. Several Phospho-S6 Ribosomal antibodies were tested in this study for various applications; the antibody reviewed here (Cat# 2211) gave the best results in Western Blot of Phospho-S6 Ribosomal (Ser 235/236) on the expression of control and patient fibroblast cells.
Western Blot
Human fibroblast cells
Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (Cell Signalling, Cat. No. 2211,incubated 2h at 4°C in Blotting-Grade Blocker (1:1000 dilution)
Blotting-Grade Blocker (BIO RAD, cat.no 170-6404)
Anti-rabbit IgG, HRP-linked antibody (Cell Signaling, cat. no. 7074), incubated 2h incubation at room temperatureC (1:5000 dilution)
NA
Amersham TM ECL Prime Western Blotting Detection reagent (GE Heathcare, cat. no. RPN2232), Image J software
This antibody worked excellently in Western blot analysis of fibroblast expression of Phospho-S6 Ribosomal Protein (Ser235/236). Only minimal background blotting control could be detected.
None
Highly specific
A little expensive
This is a very good antibody to detect Phospho-S6 Ribosomal on cell lines. The minimal background blotting is tolerable when compared to expression cells.