Novus Biologicals
Chicken anti-Escherichia coli beta-Galactosidase antibody
NB100-2045
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We had a transgenic mouse expression B-gal under the promoter for our gene of interest; we used this antibody to detect expression
Immunofluorescence
Embryonic mouse stomach
1/100 in 10% FBS PBST, 4 C overnight
10% FBS diluted in PBST
1/300 Jackson Immunoresearch Laboratories 703-486-155 Donkey anti Chicken 488
None used
Zeiss fluorescent microscope
This antibody worked well for detecting B-gal.
Signal from this antibody is in green. Blue is DAPI.
Strong signal
Some minor background
I would recommend this antibody.