Abcam
Anti-ASPA antibody
ab97454
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This antibody was used in a preclinical gene therapy study that investigated the feasibility of single intravenous injections to restore aspartoacylase in a knockout mouse model. It has been published in Molecular Therapy in 2013 [PMID: 23817205 ] Several antibodies were tested in this study including some custom made peptide antibodies as well as some made in a collaborating lab for various applications. This antibody (ab97454) gave the best results in Western blot however its immunofluorescence results were not as great.
Western blot
Brain homogenates
Aspartoacylase antibody incubated overnight at 4°C in blocking reagent (1:1000 dilution)
LiCor Odyssey Blocking Buffer (PBS) Cat#927-40000
Donkey anti-rabbit IR-conjugated antibody [LiCor cat#926-32213] incubated for 1 hour at room temperature (1:1000 dilution)
NA
Odyssey Imager was used to detect and quantify signal
This antibody worked excellently in Western blot analysis of brain homogenates of all animal groups. There was only a slight background staining which is a remarkable improvement over other homegrown antibody batches.
None
Easy; did not require extensive optimization
Did not work well for immunofluorescence
The antibody is specific for Western blot applications but is not a good choice for immunofluorescence.