Covance/Signet antibodies
CAK1 monoclonal antibody
SIG-3623
Browse Similar Products: CAK1 Monoclonal Antibodies
I used this antibody to measure mesothelin expression on different cancer cell lines. I must say that the staining is pretty good and there is almost no background staining. I performed tests with multiple cell lines and a positive control (K562) that had transgenic expression of mesothelin. Upon analysis, I could clearly see the differences between the positive and negative cells (see flow image attached). The staining had to be standardized according to cell types. Cancer cells get a lot of background staining, but by using isotype control as well as gating out doublets in FSC-W and SSC-W plots, I was able to see very specific expression. Highly recommended for flow cytometry based assays. The company advertises for immunohistochemistry, but it works for flow as well.
Flow Cytometry
K562, Capan-2 and SKOV3 cells
1:40 for 45 minutes at room temperature
None
1:250 for 30 minutes at room tempterature
NA
Flow cytometry on Alexa-fluor647 channel
K562 and Capan-2 cells show uniformly high expression of mesothelin, whereas SKOV3 cells showed two populations, one with high and one with low mesothelin expression.
Great specificity
Need a high concentration for staining; expensive
No competitors available for this antibody, so the price is high. A great product to use on Flow cytometry based applications.