Immunofluorescence by using Cytokeratin 5 antibody

December 26, 2014

Eppley instutude
University of Nebraska Medical Center
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Cytokeratin 5

Catalog Number:

PRB-160P

This antibody was used in a study that investigated effects of different EGFR Ligands on Human Mammary Epithelial Cell Differentiation. It is published in PLoS One in 2013 (Mukhopadhyay et al., Distinct Effects of EGFR Ligands on Human Mammary Epithelial Cell Differentiation. PLoS One. 4;8(10):e75907. PubMed PMID:24124521;). Many antibodies were tested but the antibody reviewed here gave the best result in Immunofluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human mammary epithelial cell

Primary Incubation

1:2000, overnight, 4C

Blocking Agent

10% goat serum

Secondary Incubation

1:1000, 1 hour, room temperature

Tertiary Incubation

NA

Detection

Confocal Microscopy

Results Summary

This antibody is very good

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Nice antibody

The Bad

Have to use specific dilution otherwise chance of getting nonspecific staining

The Bottom Line

Very good antibody

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